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Journal: Bioactive Materials
Article Title: Biomimetic de novo construction of hierarchically aligned and gradient-mineralized collagen for tendon-bone integrated regeneration
doi: 10.1016/j.bioactmat.2026.03.003
Figure Lengend Snippet: Synergistic electroassembly and post-treatment guide hierarchical collagen alignment with strong mechanics. (a) Schematic of the Hierarchically aligned collagen (HA-Col) fabrication process: the electro-assembled collagen network is aligned through mechanical prestretching with different strains to induce the alignment of internal nanofibrils, and then the stretched collagen network is further treated with ionic solution (i.e., PBS) under continuous tensile force loading to induce the aligned nanofibrils thickening and forming hierarchically aligned fibrillar microstructure with D-banding characteristics. Finally, the sample is dried for structural densification and covalently cross-linked with glutaraldehyde (1% w/v). (b) Polarized optical microscopy images of HA-Cols fabricated under different strains show a gradually increased optical birefringence with a strain-dependent manner, indicating strain-induced alignment of HA-Cols. Photos were taken in cross-polarized mode, in which the polarizer (P) and analyzer (A) were perpendicular to each other, and the sample's prestretching axis was kept at a 45° angle to the analyzer and polarizer. (c) Low- and (d) high-magnification SEM images and (e) 2D SAXS patterns (ƒ c is the Herman's orientation parameter) of HA-Cols under different strains. (f) 1D SAXS patterns confirm the formation of collagen featured D-banding in HA-Cols. (g) Stress-strain curves of the dry HA-Cols along the alignment direction and (h) the quantitative ultimate stress and Young's modulus of dried samples. Data are shown as mean ± SD (n = 3). Statistical analysis was performed using a one-way ANOVA with Tukey's post hoc test: ∗∗∗P < 0.001.
Article Snippet: Type I collagen of porcine skin origin, with its telopeptides enzymatically removed to minimize immunogenicity, was obtained from Shanghai Haohai Biotech Co., Ltd. Hydrogen peroxide, Na 2 HPO 4 ·12H 2 O, NaCl, NH 4 HCO 3 , and ethanol were procured from Shanghai Lingfeng Chemical Reagent Co., Ltd. Acetic acid and
Techniques: Microscopy